Colonisation in pregnant women is estimated to be 11 to 35%, and socioeconomic factors directly impact the percentage of carriers in the population. Streptococcus agalactiae, also known as Group B Streptococcus (GBS), is a leading cause of sepsis and meningitis in newborns and young infants. Conclusionĭata suggest that this qPCR method performs adequately as a means to detect GBS in vaginal-rectal swabs from pregnant women. It detects all strains of GBS with the same level of performance as microbiological methods. It has a limit of detection of 0.7 GE/μL and 100% analytical specificity. Our qPCR method for detecting GBS was analytically validated. The performance of this qPCR method for detecting GBS was compared to other microbiological methods used with vaginal-rectal samples from pregnant women. The methods used are described in international guidelines and other existing reports. Inclusivity, exclusivity, anticipated reportable range, the limit of detection and robustness were evaluated. All gDNA was used to evaluate the analytical validation of the qPCR method employing a specific Taqman probe. Additionally, gDNA was isolated from 21 strains of GBS from various serotypes (Ia, Ib and II-VIII). Genomic DNA (gDNA) was purified from 12 ATCC bacterial strains, two belonging to GBS and the remainder acting as negative controls. In this study, we aimed to evaluate the analytical validation of qualitative real-time polymerase chain reaction (qPCR) as a means to detect GBS. Group B Streptococcus (GBS) is the leading cause of invasive neonatal infection.

Amplifx primer list format